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單細(xì)胞轉(zhuǎn)錄組測(cè)序(Single-cell RNA-sequencing, scRNA-seq)是在單個(gè)細(xì)胞水平對(duì)mRNA進(jìn)行高通量測(cè)序的一項(xiàng)新技術(shù)，原理是將分離的單個(gè)細(xì)胞中微量的mRNA通過(guò)高效擴(kuò)增后再進(jìn)行高通量測(cè)序。

單細(xì)胞測(cè)序主要涉及單細(xì)胞基因組測(cè)序和轉(zhuǎn)錄組測(cè)序兩個(gè)方面，分別針對(duì)單細(xì)胞DNA及RNA進(jìn)行序列分析和比較。單細(xì)胞測(cè)序一直是科學(xué)家關(guān)注的一個(gè)熱點(diǎn)。

Cells, the basic units of biological structure and function, vary broadly in type and state. In most biological systems, our knowledge of cellular diversity is incomplete, as for complex tissues like the nervous system (brain cells) [1].The

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Drop-seqDroplet Based scRNA-Seq Methods